Document qdRwRxY6ddmQe25KoEXZD895M

`*<0 S7l-6*1-23 9hAmMaIoI BIO-TEST JafouitolieS, Snc. 1810 FRONTAGE ROAD NORTHBROOK, ILLINOIS 60062 Aft t A COD* IJ ttllfHONt J7-0J0 REPORT TO MONSANTO COMPANY FOUR-DAY FISH TOXICITY STUDIES ON SEVEN MATERIALS IBT NO. A7204 DSW 034293 STLCOPCB4018120 Undutkial BIO-TEST Ialmato>uei,, 9nc. 1810 FRONTAGE ROAD NORTHBROOK. IUINOIS 60062 AKIA COO! m TtltfHONI 31S-JOJO v June 13, 19^9 Mr. Elmer P. Wheeler, Manager Environmental Health Monsanto Company 800 N. Lindbergh Boulevard St. Louie, Missouri 63166 . Dear Mr. Wheeler: Re: IBT No. A7204 - Four-Day Fish Toxicity Studies on Seven Materials \ We are submitting herewith our laboratory report dated June 13, I969 prepared in connection with the above study. Very truly yours. JCC/kjl J. C. Calandra President J OSW 034294 STLCOPCB4018121 $tululVtlal B I O T E S T Ja&o'iaiotfei, 9ta, REPORT TO MONSANTO COMPANY FOUR-DAY FISH TOXICITY STUDIES ON SEVEN MATERIALS IBT NO. A7204 I. Introduction At the request of Monsanto Company, four-day fish toxicity studies in Rainbow Trout (Salmo gairdnerii) and Bluegills (Lepomls macrochirus) were conducted on the following seven materials: ' 1) Aroclor 1242, Lot 0AK-255 2) Aroclor 1254, Lot #AK-38 3) -Aroclor 1260, Lot #AK-3 1 4) Aroclor 5460, Lot #AK-390 5) Toxaphehe ' ' 6) Technical DDT (2-1/2#), B0903604 7) Halo^a^l 099, 52 percent Cl DSW 034295 STLCOPCB4018122 frtdniUtel B I O - T E S T Jaioiak'itel, UtiC. 2 II, Summary The results of the four-day fish toxicity studies are summarized below. Due to the paucity of one of the seven test materials (Halowax 1099), complete data are not available and the figures in parentheses represent only range-finding data. Test Material Four-Day LCgg (ppm) Rainbow Trout Bluegill Aroclor 1242 ~ > 100.0 0.66 Aroclor 1254 > 100.0 0.66 Aroclor 1260 . > 100.0 > 100.0 Aroclor 5460 > 100.0 . > 100.0 Toxaphene 0.0080 . 0.0056 Technical DDT 0.0052 ' 0.0078 Halowax 1099 i # (10-100) (> 100.0) Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, Report prepared by: Carmen Mastri, B.S. Section Head Acute Toxicity Department - Ji Report approved by: M, L. Keplinger, Ph.D. Assistant Laboratory Director Otu 6. June 13, 1969 Otis E. Fancher, Ph.D. Director DSW 034296 STLCOPCB4018123 HtuLiitiial B I O - T E S T Ja&yM&vrfcl, Due. 3/ 3 III. Procedure The procedure was generally the same for both bluegills and rain bow trout with each test material. Healthy, young fish with an average length of 35 to 75 millimeters were used as test animals. All fish were kept under observation for a period of not less than ten days prior to experimental use. During this period, the fish were observed for general health and suitability as test animals. The fish were held in large aerated stock tanks and were fed frozen brine shrimp until three days immediately prior to testing. The rainbow trout were maintained at a temperature of 13C and the bluegills at a temperature of 24C. All stock tanks and bioassay vessels contained reconstituted, deionized water. The following compounds were added in the amounts stated per liter of deionized water: 30 mg calcium sulfate r 30 mg magnesium sulfate ` 48 mg sodium bicarbonate 2 mg potassium chloride Preliminary screening was conducted in order to find the general level of toxicity before beginning the actual bioassay. Once this level had been determined one to five concentrations were selected for the actual bioassay. Bioassay vessels lined with disposable polyethylene bags were filled with ten liters of well aerated reconstituted water. To evaluate the selected concentrations, ten trout were placed in each bioassay vessel. In the case of the bluegills only five fish were placed in each bioassay vessel in order to insure adequate dissolved oxygen concentration (D.O.) DSW 034297 STLCOPCB4018124 DnduiUlal B I O T E S T J.abo\ai/yilcA, D*i&. 4 in the warmer water; therefore two groups of five bluegills were tested at each selected concentration. An untreated control group consisting of 10 fish was also employed. After a 24-hour acclimatization period, calculated amounts of test material necessary to produce the desired one to five concentration levels were dispensed into the respective vessels. The test solutions were prepared within one hour of the bioassay. - - Following the introduction of the test material the fish were observed for 96 hours during which time all mortalities and/or untoward behavioral reactions were recorded. The concentration of dissolved oxygen was de termined for all test solutions in which mortalities occurred, to insure that adequate oxygen was available to the test specimens*. Concentrations of dissolved oxygen of 4 mg/1 (4 ppm) for warm water fish (bluegill) and 5 mg/1 (5 ppm) for cold water fish (trout) were considered to be minimal v for valid testing. The pH of the test solution was also determined when - mortalities were recorded. At the end of the observation period all data were collected and ar rangements were made to calculate the 96-hour LCjq employing the technique of Litchfield and Wilcoxon**. * Standard Methods for the Examination of Water and Wastewaters, Twelfth Edition, American Public Health Association, Inc. , 1790 Broadway, New York, N, Y.: Part III, pp. 406-410. ** J. T. Litchfield, Jr. and F. Wilcoxon, "A Simplified Method of Evaluating Dose-Effcct Experiments," J. Pharm. k Exp. Thcr. , Vol. 96, No. 2, pp. 99-113. OSW 034298 STLCOPCB4018125 Qndirtklol BIO-TEST J!al&\aUilcl, 9hc* Zt 5 IV. Results No deaths or untoward behavioral reactions were noted among any of the animals in the untreated control groups. A. Rainbow Trout 1. Aroclor 1242, Aroclor 1254, Aroclor 1250, Aroclor 5460, Toxaphene, Technical DDT, Halowax 1099* ~~ a. Mortality, DO, and pH Data r . The mortality, dissolved oxygen content, and pH data are presented in Tables I through IV. __ ... .. I * Range finding mortality only. DSW 034299 STLCOPCB4018126 SruhiSiHul D IO -T O S T J!a&yu2h>ih'S, Stic, STLCOPCB4018127 TEST MATERIAL: Aroclor 1242, 1260^^60^)1254 Four-Day Fish Toxicity Study - Rainbow Trout Mortality, DO, and pH Data , Number Dead Total Per Dissolved Oxygen (ppm) and pH Concentration* Hours: Number Number Cent 1-6 Hr, 24 Hr, 48 Hr. 72 Hr. 96 Hr. Test Materialfppm)1-6 24 48 72 96 Dead Tested Dead DO pH DO pH DO pH DO pH DO pH Aroclor 1242 100.0 0 0 0 0-0 10 Aroclor 1254 Aroclor 1260 Aroclor"546<f 100.0 100,0 0 0 00 0 10 0 0 0 00 0 10 0 A0 A A ../(T ;jC2To--------- TO---~Q s' -:-XT Pour-Day LC50 Aroclor 1242 > 100.0 ppm Aroclor 1254 > 100.0 ppm Aroclor 1260 >_100. 0 ppm "Aroclor 5460 >.200, 0 ppm)7) ' ': ; ; . * The test materials were dispensed into the bioassay vessels in the form of a 10.0 percent (w/v) solution in acetone. Concentrations are expressed in terms of actual test material as received. . c Co X o OJ o o O' (u w tf `W tv/D nyxy i S 3 l - O 1 fl TABLE H TEST MATERIAL: Toxaphene Four-Day Fish Toxicity Study - Rainbow Trout; Mortality, DO, and pH Data Number Dead Total neent ration^ Hours: Number Number (ppm) 1 ~6> 24 48 72 96 Dead Tested ' Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH ' 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH 0.0056 00000 0 10 0 0.010 0.018 0.032 0,056 09 09 04 0 10 000 1- 6-- * 9 10 10 10 10 10 10 /' 10 90 100 100 . 100 - - 8.2 7.4 - -- 7.6 8.0 7.4 - - - 7.8 6.9 8.1 7.0 - - - 8,1 7.2 m -- -- -* . - - _ .ur-Day LC50 = 0. 008 ppm . /4 , The test material was dispensed into the bioassay vessels in the form of a 0,01 percent (w/v) solution in acetone. Concentrations are expressed in terms of actual Toxaphene as received. ru 00 s a STLCOPCB4018128 a</! oU) < UoJ 69 TABLE m ' TEST MATERIAL: Technical DDT Four-Day- Fish Toxicity Study - Rainbow Trout '' Mortality, DO, and pH Data Number Dead Total :cntrationS Hours: Number Number (ppm) 1-6 24 48 72 96 Dead Tested Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH ' 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO PH DO pH DO pH DO pH 0.001 000 0 0 0 10 0 -- 0.0013 0 0 0 0' 0 0 10 0 0. 0032 0.0056 0.01 0 0 0' 0 0 0 0 4' 1 2 063 1 - 0 7 10' 10 10 10 ' 0 70 100 -' --- --- - '- -- - - 8.0 7.3 7.8 7.2 8.4 7.2 8.2 7.3 8.0 ' 7.2 7.9 7.2 - - -Day LC50 = 0. 0052 ppm ; ie test material was dispensed into the bioassay vessels in the form of a 0, 001 percent (w/v) solution in acetone, jncentrations are expressed in terms of actual Technical DDT as received. TABLE TV . TEST MATERIAL: Halowax 1099 Range Finding Fish Toxicity Study - Rainbow Trout . Mortality, DO, and pH Data Number Dead Total * it rat ion* Hours: Number Number pm) 1-6 24 48 72 96 Dead Tested Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH ' 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH DO, 0 00002 2 2 100 - - 10.0 00 00 0 0 2 0 -- --- -- -- - Day LC50 = 10-100 ppm : test material was dispensed into the bioassay vessels in the form of a 10,0 percent (w/v) solution in acetone, icentrations are expressed in terms of actual Halowax 1099 as received. ' huiuiUlat BlO-T E ST J!akyiaio4lc4, Puc. 10 b. Reactions . ' The reactions noted following introduction of the Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460, Toxaphcne, and Technical DDT are presented in Tables V through X respectively. DSW 034304 STLCOPCB4018131 0P tu ta lklo l B I - T E S T lal&iato'U ci, Snc. STLCOPCB4018132 TABLE V ' TEST MATERIAL: Aroclor - 1242 Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Time of Onset Duration Time of Death Following Dose of Following Dose Concentration Administration Reaction Administration _ (PPm? Reaction (hours) (hours) (hours) 100.0 Hypoactivity # 24 No Recovery Noted - Inverted Swimming 72 No Recovery Noted - j j i oCaco obJ W o U 0B I T EST la lo ta L v ih i, 9ttc. TABLE VI 'TEST MATERIAL: Aroclor - 1254' Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Concentration (ppm) 1 Reaction 100.0 No Reactions Noted Time of Onset Following Dose Administration (hours) - Duration of Reaction (hours) -' Time of Death Following Do3e Administration (hours) - o Co o OJ w C/> o o O O o O DO oU 00 CO ih , Pftdiiiltiol B 1O - T E ST XaloiatyilM, Ate. STLCOPCB4018134 TABLE VII TEST MATERIAL: Aroclor 1260 Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Concentration (ppm) 100.0 Reaction Hypoactivity Time of Onset Following Dose Administration {hours) Duration of Reaction (hotirs) Time of Death Following Dose Administration (hours) 72 No Recovery Noted D CO X o UJ o 1v wy tv w fv w jo f XSa X I 30 TABLE vm TEST MATERIAL: Aroclor - 5460 Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Concentration {VPTM) ..... _ Reaction 100. 0 No Reactions Noted Time of Onset Following Dose Administration (hours) '- Duration of Reaction (hours) - Time of Death Following Dose Administration (hours) - o Co X o U) S' o 03 w STLCOPCB4018136 TABLE IX TEST MATERIAL: Toxaphene . Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Concentration (ppm) Reaction 0-. 0056 No Reactions Noted 0. 010 Hypo activity 0. 018 Hyperactivity , 0. 032 Hyperactivity 0. 056 Hyperactivity Time of Onset Following Dose A dmini a tration {hours) Duration of Reaction (hours) Time of Death Following Dose Administration (hours) - -- 24 24 24 ' 24 Until Death 24-48 . 24 Until Death 24-48 1-6 . Until Death 24 / o C/o sc o u> o SO s 3 1 - o i ai VI w STLCOPCB4018137 TABLE X TEST MATERIAL: Technical DDT Four-Day Fish Toxicity Study - Rainbow Trout Summary of Reactions Concentration (ppm) Reaction Time of Onset Following Dose Administration (hour3) Duration of Reaction (hours) Time of Death Following Dose Administration (hours) 0. 001 No Reactions Noted -- 0. 0018 No Reactions Noted - '' " - 0. 0032 0. 0056 No Reactions Noted / Hyperactivity ' . 1-6 . " No Recovery Noted 48-72-96 0.01 Hype ractivity Prostration 1-6 '4 Until Death 24-48-72 o (/> SE o 0>-' $0J o s 3 x " 0 1ai o f. ftuhulAlal B I 0 - T E 5 T 2a!&>iat>Mc&, 9hc. J 17 B. Blucgills 1. Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460 Toxaphene, Technical DDT and Halowax 1099* a. Mortality, DO, and pH Data . The mortality, dissolved oxygen content, and pH data are presented in Table XI through XVII respectively. I * Range finding mortality only. DSW 034311 STLCOPCB4018138 STLCOPCB4018139 . 1 i ' TABI/E XI TEST MATERIAL:'Arodor 1242 Four-Day Fish. Toxicity Study - Blue gill Mortality, DO, and pH Data Number Dead Total ' :cntration* Hours: Number Number (ppm) 1-6 24 48 72 96 Dead Tested PerCent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Kr. DO pH DO pH DO pH DO pH 0.10 000000 10 0 .......................................... ........................................................ 0.18 00000 0 10 0 - - - -- -- -- 0.32 00 000 0 10 0 - - - -- -- -- - 0.56 000 0. 0 0 10 0 l.o 0 10 - - - 10 10 100 - - 7.6 7.2 - -- -- - r-Day LC50 = 0. 66 ppm ` he test material was dispensed into the bioassay vessels in the form of a 1.0 percent (w/v) solution in acetone, oncentrations are expressed in terms of actual Aroclor 1242 as received. D V) s: o * OJ *"> W tf `tw y e u y v f 1 s 3 - O I (!I CO l*  TABLE XU - TEST MATERIAL: Aroclor 1254 Four-Day Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead Total rcntration* Hours: Number Number (ppm) 1-6 24 48 72 96 Dead Tested Per Cent. 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH 0. 10 0.18 0.32 0.56 1.00 00000 *. 000p0 000 00 0 0 0 0- 0 0. 0 6 3 1 0 0 0 0 10 10 0 10 0 -_ " 4 10 0 -- ** - - . - - -- - 10 0 10 100 - - .7,8 7.2 6.5 7.2 6.2 7.1 r-Day LC5Q = 0,66 ppm ` he test material was dispensed into the bioassay vessels in the form of a 1.0 percent (w/v) solution in acetone, oncentrations are expressed in terms of actual Aroclor 1254 as received, o <ast> o oj . UJ SL C3 O m M --4 r 0 xwf twojvhyp/- J. S 3 " O iI TABLE xnr TEST MATERIAL: Aroclor 1260 Four-Day Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead Total nccntration* Hours: Number Numbe r (ppm) 1-6 24 48 72 96 Dead Tested -------------------------------------------------------------------- 1__ Dissolved Oxygen (ppm) and pH Per Cent 1-6 Hr. 24 Hr- 48 Hr. 72 Hr. 96 Hr. Dead DO pH DO pH DO pH DO pH DO pH 100,0 0000 0 0 ' 10 0 -- ar-Day LC5q> 100,0 ppm . the test material was dispensed into the bioassay vessels in the form of a 10, 0 percent (w/v) solution in acetone, Concentrations are expressed in terms of actual Aroclor 1260 as received, - o in X o (/) OJ t-* S' r-j o b, 'w to/w ye/ X S3 X- O HI TABLE XTV TEST MATERIAL: Aroclor 5460 Four-Day Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead snccntration* Hours: (ppm) 1 -6 24 48 72 Total * Number Numbe r 96 Dead Tested Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH 100.0 00000 0 10 0 -- jur-Day LC5q> 100, 0 ppm '. The test material was dispensed into the bioassay vessels in the form of a 10,0 percent (w/v) solution in acetone. Concentrations are expressed in terms of actual Aroclor 5460 as received. STLCOPCB4018142 o/ C/i sc o w> (JJ tvn t\l u. I `fvrto/ei.xyvf S 3 X O 1 8i TABLE XV / TEST MATERIAL: Toxaphene i Four-Day Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead Total iccnt ration^ Hours: Number Number . (ppm) 1 -6 24 48 72 96 Dead Tested Per Cent Dead 1-6 Hr. DO pH .............. - ....... ................. ....... . 1 Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH 0.0010 0.0018 0.0032 00000 * 00000 00000 0 0 0 10 10 10 0 0. 0 -- -- - -- -- r* 0.0056 0 ' 0 5 0. 0 5 10 50 ' - 7.3 7.2 - -- - 0.01 0070 1 8 10 . 80 -- - - 7.3 7.2 - - 6.1 7.2 r-Day LC50 = 0. 0056 jercent Confidence Limits of LC50 d.0036 - 0.0087 ppm :he test material was dispensed into the bioassay vessels in the form of a 0.01 percent (w/v) solution in acetone. Concentrations are expressed in terms of actual Toxaphene as received. o </> X O U) ^' u> * a - ' ' ts ts I I I JPh u mo B l O - T E ST c STLCOPCB4018144 . ' TABLE XVI. TEST MATERIAL: Teclinical DDT Four-Day Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead Total cent ration^ Hours: Number Number (ppm} 1 -6 24 48 72 96 Dead Tested Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH ` 0.001 00000 0 10 0 -- 0,0018 9 .0 0 0 0 0 . 0 10 0 0.0032 0 00 0 0 0 10 0 -- -m -- -- -' 0.0056 0.01 0 0 0 0. 0 0 10 - - - 0 - 10 10 ' 0' -100 - - 7.6 7.1 - _- -- - -Day LC50 0,0078 ppm .. le test material was dispensed into the bioassay vessels in the form of a 0.001 percent (w/v) solution in acetone, mcentrations are expressed in terms of actual Technical DDT as received. . o </) X o UJ pUf--) ts> *sl SnJhtihlal B IO -T E S T J.ahy\aU>d&i, Hm , TABLE XVH TEST MATERIAL: Halowax' 1099 Range Finding Fish Toxicity Study - Bluegill Mortality, DO, and pH Data Number Dead Total ' cntration# Hours: Number Number ppm) 1-6 24 48 72 96 Dead Tested Per Cent 1-6 Hr. Dead DO pH Dissolved Oxygen (ppm) and pH 24 Hr. 48 Hr. 72 Hr. 96 Hr. DO pH DO pH DO pH DO pH 100.0 00000 0 2 0 ........................................................................................................ 10.0 00000 0 2 0 - ---------- -Day LC50 > 100. 0 ppm e test material was dispensed into the bioassay vessels in the form of a 10. 0 percent (w/v) solution in acetone, ncentrations are expressed in terms of actual Halowax 1099 as received. c to SE O u> 0J p~* </) CD O O -0 o 00 o co tv Vi r. UrtiLiii'Ual BIO-TEST Sttc. 25 b. Reaction The reactions noted following introduction of Aroclor 1242, Aroclor 1254, Aroclor 1260, Aroclor 5460, Toxaphene, and Technical DDT are presented in Tables XVIII through XXIII respectively. DSW 034319 STLCOPCB4018146 STLCOPCB4018147 TABLE XVni TEST MATERIALS Aroclor - 1242 Four-Day Fish Toxicity Study - Bluegill Summary of Reactions Concentration (pom) Reaction 0.1 No Reactions Noted 0.18 No Reactions Noted 0.32 0.56 Hype ractivity Hyperactivity / 1.0 Hyperactivity ' Time of Onset Following Dose Administration (hours) - -' 1-6 1-6 1-6 Duration of Reaction (ho\irs) - Time of Death Following Dose Administration (hours) . - -- . 24 - 24 ' - Until Death 24 o</> sc O' b) bN)) O l . . `rm syw iyo f X s 3 l " 0 I 8 W " / ' . cr* fc 0S /u fu ilu a l B 1 - T E S T Xal&iabyUcl, Stic, STLCOPCB4018148 TABLE XIX TEST MATERIAL: Aroclor - 1254. Four-Day Fish Toxicity Study - Bluegill Summary of Reactions Concentration (ppm) Reaction 0.1 No Reactions Noted 0.18 No Reactions Noted 0.32 No Reactions Noted , 0.56 Hyperactivity Hypoactivity 1.0 Hypoactivity Time of Onset Following Dose A dmini a t r ation (hours) -' - -. .1-6 24 '2.4 Duration of Reaction (hotirs) - Time of Death Following Dose Administration (hours) - -- -. - 24 ' - ' 48 * Until Death 48-72-96 / o (XS, o OJ ONJ) t v w y w je ? JLS31 * 0 I a TABLE XX TEST MATERIAL: Aroclor - 1260. t ) ( Four-Day Fish Toxicity Study - Bluegill I Summary of Reactions Concentration ' (PPm> Reaction 100.0 Hypoactivity , I` ' Time of Onset Following Dose Administration (hours) Duration of Reaction, (hours) Time of Death ' Following Dose Administration (hours) 1 ' 48 No Recovery Noted 1 c {/o O OJ r u) rv> 00 STLCOPCB4018149 ; i ;' TABLE XXI TEST MATERIAL; Aroclor - 5460 Four-Day Fish Toxicity Study - Bluegill . Summary of Reactions Concentration (ppm) Reaction 100.0 Hypoactivity Time of Onset Duration Following Dose of Administration Reaction (hours)____________(hotirs) Time of Death Following Dose Administration ______ (hours) 72 . No Recovery Noted w 03 0 -i m 1 t r a (/> s: o OJ KJ U) / / ts O STLCOPCB4018150 TABLE XXII TEST MATERIAL: Toxaphene ` Four-Day Fish Toxicity Study - Bluegill ' Summary of Reactions Concentration (ppm) Reaction 0. 001 No Reactions Noted 0. 0018 No Reactions Noted 0.0032 No Reactions Noted 0.0056 Hypoactivity 0.01 Hypoactivity Prostration Loss of equilibrium Time of Onset Following Dose Administration (hours) Duration of Reaction (hours) Time of Death Following Dose Administration (hours) -' l ' - ' '' - - - -* ' ' 48 24 . 48 ' 24 * 24 ' 48 - Until Death 48-96 .TABLE XXIII TEST MATERIAL: Technical DDT Four-Day Fish Toxicity Study - Bluegill Summary of Reactions Concentration (ppm) Reaction Time of Onset Following Dose Administration (hours) Duration of Reaction (hours) Time of Death Following Dose Administration (hours) 0.001 0.0018 No Reactions Noted No Reactions Noted '' - - .- .- -' - 0.0032 No Reactions Noted - _- - 0.0056 ' No Reactions Noted 0. 01 Hype rac tivity, ' .' 1-6 f Until Death 24 s vs O m tr r ion si (/> 4oUs> oooo U) IUS)l DO 0 00 01 Is) / v 0.